indigenous medicinal plants

The interviews were followed by the collection of plants under the supervision of three traditional healers. USA.gov. Chemicals used in the assay were purchased from Sigma and Aldrich chemicals, UK, and Ciprofloxacin was purchased from DENK PHARMA GmbH & Co, Germany. It was then eluted with gradient mixtures of pet-ether, EtOAc, and MeOH. The vernacular names of plants, uses, methods of preparation, and the administration of remedies were documented. Majeed M, Bhatti KH, Amjad MS, Abbasi AM, Bussmann RW, Nawaz F, Rashid A, Mehmood A, Mahmood M, Khan WM, Ahmad KS. In this study, medicinal plants traditionally used for the treatment of infections in the Ejisu-Juaben district have been documented through an ethnobotanical survey. This is the time to sweat more and breed less as we research more on indigenous medicinal plants for yield of innovations and inventions before the knowledge holders disappear to … 2005; 100: 43–49. J Ethnopharmacol. Its medicinal values are: Medicinal plants for gastrointestinal diseases among the Kuki-Chin ethnolinguistic groups across Bangladesh, India, and Myanmar: A comparative and network analysis study. J Ethnobiol Ethnomed. J Ethnopharmacol. Flavonoids, fatty acid esters, and trichothecenes have also been reported [29–31]. Indigenous knowledge of medicinal plants used by the Reang tribe of Tripura state of India J Ethnopharmacol. Herbal medicines are highly valued and enjoy patronage by a large number of the populace due to its acceptability, affordability, and perceived safety [9]. Medicinal plants form the basis of traditional or indigenous health systems used, in the estimate of the World Health Organization, by the majority of the population of most developing countries. This work was supported by the Building Stronger Universities (BSU) Grant, WP3. HHS Epub 2014 Jan 7. The antibacterial activity demonstrated by the crude extract and the solvent fractions of the H. floribunda stem bark in the current work is consistent with previous reports on the same plant [22–26]. All participants provided information on the methods of practice, vernacular names of anti-infective plant remedies employed by them, methods of preparation, and administration of these remedies. eCollection 2020. Lyonia. The raw data/results from experiments used to arrive at the findings of this study are available from the corresponding author upon request. To ensure cellular quiescence, this dilution was maintained at 4°C. They were then stored in a cool dry place until required for use. 2020, Article ID 8821905, 10 pages, 2020. https://doi.org/10.1155/2020/8821905, 1Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana, 2Department of Biomedical Sciences, School of Allied Health Sciences, University of Cape Coast, Cape Coast, Ghana. H. floribunda crude extract (MHF), which showed the highest biofilm formation inhibition toward S. aureus (Figure 4(a)), was successively fractionated to obtain the pet-ether (PHE), ethyl acetate (EHE), and methanol (MHE) fractions, and their biofilm inhibitory effects were investigated. Demographic data of local informants…. and Schinz: holarrheline, holadienine, holaromine and holaline,”, K. Appiah, C. Oppong, H. Mardani et al., “Medicinal plants used in the Ejisu-Juaben municipality, Southern Ghana: an ethnobotanical study,”, N. K. Archer, M. J. Mazaitis, J. W. Costerton, J. G. Leid, M. E. Powers, and M. E. Shirtliff, “, A. M. Lannang, S. Anjum, J. G. Tangmouo, K. Krohn, and M. I. Choudhary, “Conessine isolated from. The resulting solution was made distinctly alkaline with 70 mL of strong ammonia. Indigenous medicinal plants are relevant in both developing and developed nations of the world as sources of drugs or herbal extracts for various chemotherapeutic purposes ( Purbrick 1998 ; Farombi 2004 ). Withania somnifera and Ranunculus sceleratus with maximum FL (100%), were used against gastrointestinal and urinary disorders, respectively. A negative control was included and used to calculate a cutoff OD value, which separates a nonbiofilm producer from a biofilm-producing organism according to the established standards [11,14]. HF1A, HF1B, and HF1C are isolated compounds. The wells were then washed with sterile water and dried. 26. Biofilm formation was significantly mitigated by petroleum ether, ethyl acetate, and methanol extracts of Holarrhena floribunda stem bark. Sign up here as a reviewer to help fast-track new submissions. Slides were then stained with 0.1% crystal violet for 15 minutes, washed with distilled water, air dried, and observed under the microscope. 1997). (a), Photomicrographs of adhering bacterial cells (biofilms) stained with 0.1% crystal violet on a glass slide after 24 hours, observed at ×100: (a). Indigenous uses of medicinal plants in North Garo Hills, Meghalaya, NE India Sharma M. * , Sharma C.L. Zahoor M, Yousaf Z, Aqsa T, Haroon M, Saleh N, Aftab A, Javed S, Qadeer M, Ramazan H. J Ethnobiol Ethnomed. After incubation, the planktonic cells were aspirated and the wells were washed with sterile water to remove the free-floating bacteria. Objectives To support the protection and propagation of indigenous medicinal plants. These data can serve as evidential support for the clinical development of a number of medicinal plant remedies as adjuvant therapy. Ethnobotanical data were analyzed using quantitative tools, i.e. 2000; 287: 44–45. This is however the first report of the biofilm formation inhibitory effect of H. floribunda and the isolated plant constituents. Further in this study, the biofilm formation inhibitory effect of selected medicinal plants against biofilm-producing Staphylococcus aureus was investigated. Kabo khaji Katha Mimosaceae HD 2 Linn. 2019 Feb 1;15(1):7. doi: 10.1186/s13002-019-0285-4. In a previous study, the hydroalcoholic extract H. antidysenterica inhibited the formation of biofilm in opportunistic pathogenic Salmonella typhimurium [41]. Environmental Service Facebook is showing information to help you better understand the purpose of a Page. Specialists could administer such medicines as herbal teas, preparations to be chewed and swallowed, poultices, inhaled vapours, or a variety of other applications. Fig 2. In the rural and peri-urban areas like the Ejisu-Juaben district in the Ashanti region of Ghana, access to medical care is limited, and most indigenes rely on herbal medicine practitioners for their primary health needs. A cutoff OD was determined from the negative control and used to separate nonbiofilm from biofilm-producing microorganisms following standard methods [11]. A. Badmus, O. E. Ekpo, F. Rautenbach, J. L. Marnewick, A. Conessine (HF1C) is a well-established constituent of H. floribunda [19]. Among families Lamiaceae dominated over other families by donating 08 medicinal plant species followed by Apiaceae (07) and Asteraceae (06). Detail about the number of cited plants from the respective plant family is presented in Fig. Medicine in your backyard: How Indigenous peoples have used medicinal plants A program available at Wanuskewin Heritage Park shows people plants … 2002; Fact Sheet. Ciprofloxacin was used as the reference drug. Epub 2020 Jan 7. A prior informed consent form (translated into the local dialect) was given to the participants to sign after the objectives of the study had been explained. The inclusion criterion for the survey was that the practitioner used herbal medicine. Amjad MS, Zahoor U, Bussmann RW, Altaf M, Gardazi SMH, Abbasi AM. This traditional family apprenticeship is called ukushiywa nezikhwama zenyangameaning the tradition of ‘inheriting medicinal bags’ which refers to a small bag called Indigenous knowledge of medicinal plants from Gujranwala district, Pakistan. Trends in ethnopharmacology. Amjad MS, Qaeem MF, Ahmad I, Khan SU, Chaudhari SK, Zahid Malik N, Shaheen H, Khan AM. The chloroform extract was concentrated by evaporation on a rotary evaporator to yield 52.4 g of crude alkaloidal fraction. traditional health practioners, IP. Fig 1. After the incubation, the liquid suspension was removed and the amount of biofilm formed determined as described in Section 2.5.4. was employed with some modification. 271. The communities are well known for their traditional beliefs and the use of plants for medicine (Ghana Statistical Service, 2014). Ethical approval for the study was obtained from the chiefs of the studied areas and the GNATH. 2020 Oct 30;15(10):e0240555. Extracts that showed antibacterial activity were tested for biofilm inhibitory activity, and the most active plant was subsequently purified to obtain the active constituents. Three extracts, pet-ether (PHE), ethyl acetate (EHE), and methanol (MHE) fractions, were prepared from the crude extract and tested for biofilm inhibitory activity. The use of antimalarial plants as traditional treatment in Papua Island, Indonesia. 2020 Apr 6;251:112415. doi: 10.1016/j.jep.2019.112415. Seventy plants used by the traditional practitioners for the treatment of skin, wounds, and other infections were documented from the survey (Supplementary Material-Table S1). He comes from a family with a long tradition of herbalists (amaXhwele) where indigenous knowledge of medicinal plants was passed on from one generation to the next. Details of HPLC experimental procedures are provided in supplementary data (available here). Although the antibiofilm inhibitory property of H. floribunda and its constituents was not previously investigated, a recent study demonstrated the resistance-modifying ability of conessine through the inhibition of multidrug efflux pumps [35–38]. Ethno-medicinal uses of vertebrates in the Chitwan-Annapurna Landscape, central Nepal. They were authenticated according to their respective features on selective media, maintained in 30% glycerol broth and stored at −40°C in a frost-free freezer. The names and the authenticity of the plant species were confirmed using the Electronic Plant Information Centre (ePIC, http://epic.kew.org/). Medicinal plant species with high RPL values should be screened for comprehensive phytochemical and pharmacological studies. The reported ailments were classified into 11 disease categories based on ICF values and highest number of plant species was reported to treat dermatological and gastrointestinal disorders. Approximately 3,000 species are used by an estimated 200,000 indigenous traditional healers (Van Wyk et al. Among the isolated compounds, HF1A had the highest activity with an MBIC of 0.25 mg/mL toward S. aureus. Eight out of the forty plants exhibited antimicrobial activities as determined by the zones of inhibition of susceptible microbes (Table 1). For all assays, the working culture was prepared by standard dilution in distilled water and the bacterial suspension was adjusted to a turbidity matching that of 0.5 McFarland Standard (containing ∼108 colony forming units (CFU)/mL) according to the CLSI guidelines [10,11]. Fifty-six fractions were collected in 60 mL aliquots and bulked together into six subfractions based on their TLC profiles coded CF 1–6. Geneva. The isolated compounds demonstrated varying degrees of biofilm formation inhibitory properties. -. Elikplim Kwesi Ampofo, Isaac Kingsley Amponsah, Evelyn Asante-Kwatia, Francis Ackah Armah, Philip Kobla Atchoglo, Abraham Yeboah Mensah, "Indigenous Medicinal Plants as Biofilm Inhibitors for the Mitigation of Antimicrobial Resistance", Advances in Pharmacological and Pharmaceutical Sciences, vol. Traditional indigenous medicinal plants The sacred medicines Tobacco, sage, cedar, and sweetgrass are some of the fundamental and most important traditional medicines of … Also known as the boegoe plant, buchu is used in different forms as a medicine plant, either when making tea or crushed and mixed in vinegar. This work has provided comprehensive data on the medicinal plants used in the treatment of infection in Ghana. The agar well diffusion method as described by Jorgenson et al. Some extracts of neotropical rainforest plants have shown remarkable biofilm inhibitory effects [1,6]. All the traditional healers interviewed belonged to the Akan tribe. The survey data were analyzed using the Statistical Package for Social Sciences (SPSS) version 22.0 for windows and Graph Pad Prism (Version 5 for windows, San Diego, USA). One hundred and fifty grams each of the forty selected plants were dried in the shade for 7 days, milled into a coarse powder using a warring blender, and extracted with 80% methanol by cold maceration. A … Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. eCollection 2020 Dec. J Ethnobiol Ethnomed. She was told to boil the pitch of the lodgepole pine (Pinus contorta)in water and drink the in… Forty medicinal plants cited more than five times by 20% of the traditional healers were investigated for antibacterial activity by the agar diffusion assay. S. aureus was therefore selected for the investigation of biofilm formation inhibitory property of H. floribunda. COVID-19 is an emerging, rapidly evolving situation. The biofilm formation inhibitory effect of Holarrhena floribunda and its constituents validates the ethnomedicinal use of the plant in infectious disease treatment and points to the possible presence of other potentially effective antibacterial constituents in the plant. Introduction The role played by indigenous knowledge on conservation of medicinal plants is extensively acknowledged [1] [2]. Methods: general experimental procedures. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Minimum biofilm inhibitory concentrations of fractions, isolated compounds HF1A, HF1B, and HF1C, and the reference drug against, Steroidal alkaloids from the stem bark of, 6 (a-b) Graphs showing the effect of different concentrations of fractions and isolated compounds on the amount of biofilm formed (optical density-OD) by, Indigenous Medicinal Plants as Biofilm Inhibitors for the Mitigation of Antimicrobial Resistance, Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana, Department of Biomedical Sciences, School of Allied Health Sciences, University of Cape Coast, Cape Coast, Ghana, Advances in Pharmacological and Pharmaceutical Sciences, Minimum biofilm inhibitory concentration (MBIC) (mg/mL), C. M. Romero, C. G. Vivacqua, M. B. Abdulhamid et al., “Biofilm inhibition activity of traditional medicinal plants from Northwestern Argentina against native pathogen and environmental microorganisms,”, R. M. Donlan, “Role of biofilms in antimicrobial resistance,”, M. Jamal, W. Ahmad, S. Andleeb et al., “Bacterial biofilm and associated infections,”, R. M. Donlan, “Biofilm formation: a clinically relevant microbiological process,”, R. Wolcott, J. W. Costerton, D. Raoult, and S. J. Cutler, “The polymicrobial nature of biofilm infection,”, B. Sasirekha, D. Megha, M. S. Chandra, and R. Soujanya, “Study on effect of different plant extracts on microbial biofilms,”, J.-F. Hu, E. Garo, M. G. Goering et al., “Bacterial biofilm inhibitors from Diospyrosdendo,”, S. M. Bhola, F. M. Alabbas, R. Bhola et al., “Neem extract as an inhibitor for biocorrosion influenced by sulfate reducing bacteria: a preliminary investigation,”, R. M. Gyasi, A. Pakistan has a great diversity in medicinal flora and people use these ethno-medicines to deal with many skin problems. The Ejisu-Juaben district is one of the 27 administrative and political districts in the Ashanti Region of Ghana. Indigenous Medicinal Plants. Forty medicinal plants cited more than five times by 20% of the traditional healers (Percentage of respondents with the knowledge about the use of a particular plant) were investigated for antibacterial activity (shown by asterisk ‘’on Table S1, Supplementary Material). The test was carried out in 96-well microtiter plates. Fig 2. 2014 Feb 27;152(1):135-41. doi: 10.1016/j.jep.2013.12.037. doi: 10.1016/j.heliyon.2020.e05562. Contamination of Medicinal Plants: Implications for Indigenous Health A new study will trace the legacy of uranium mining on commercially available medicinal plants. 1. They were filtered, concentrated on a rotary evaporator, and dried on a water bath. MHE showed the highest biofilm formation inhibitory effect with an MBIC of 0.0625 mg/mL (Table 3). The plants were distributed across 63 genera belonging to 31 families, mostly Fabaceae (9), Euphorbiaceae (6), Moraceae (6), and Meliaceae (6). Ethnomedicinal uses of the local flora in Chenab riverine area, Punjab province Pakistan. An alkaloidal fraction (AKL) was further prepared from MHE and purified by column and high-performance liquid chromatography to yield the compounds HF1A, HF1B, and HF1C (Figure 5). Xinhua.com | Aussie researchers tap native medicinal plants in major agribusiness project NLM The rank order of biofilm-producing ability was S. aureus > S. pyogenes > E. coli > P. aeruginosa > K. pneumoniae. We have created a list of significant indigenous species that meet the health-promoting criteria, including but not restricted to plants with medicinal, aromatic and nutritional benefits. Briefly, fresh Mueller Hinton broth (100 μL) was seeded with 10 μL of standardized microorganism culture in a 96-well microtiter plate and incubated at 37°C for 24 hours. Competing Interests: The authors have declared that no competing interests exist. Meanwhile, the methanol extracts of two plants, Grossera vignei (MGV) and Holarrhena floribunda (MHF), showed appreciable biofilm formation inhibitory activities. Resistant clinical strains of Staphylococcus aureus ATCC 25923 (A), Streptococcus pyogenes clinical strain (B), Escherichia coli ATCC 25922 (C), Pseudomonas aeruginosa ATCC 27853 (D), and Klebsiella pneumonia clinical strain (E) were obtained from the Department of Pharmaceutical Microbiology, Faculty of Pharmacy and Pharmaceutical Sciences, KNUST, and used for the study. Source: Instagram. Review articles are excluded from this waiver policy. Forty of these, cited more than five times by 20% of the traditional healers, were collected from the Ejisu-Juaben district for further investigation. The petri dishes were allowed to stand for 1 hour at 25°C to allow for diffusion of extracts into the seeded agar and then incubated at 37°C for 24 hours. eCollection 2020. 2020 Oct 27;16(1):65. doi: 10.1186/s13002-020-00417-w. See this image and copyright information in PMC. They were authenticated by Dr. George Henry Sam of the Department of Herbal Medicine. This was first determined qualitatively from photomicrographs of glass slides with adhering bacterial biofilms stained with 0.1% crystal violet (Figure 3). Compounds HF1A, HF1B, and HF1C (Figure 6) were identified based on comparison of mass spectral, 1H and 13C NMR data to published literature as holonamine, holadienine, and conessine, respectively [15,16]. The categories of practitioners included diviners (17%), herbalists (67%), clairvoyants (3%), fetish priests (10%), and bone setters (3%). Proportional contributions of plant part…. Bioassay-guided fractionation of the bioactive alkaloidal fraction revealed the presence of the steroidal alkaloids, holonamine (HF1A), holadienine (HF1B), and conessine (HF1C), which also showed considerable antibiofilm forming activity. Map of Ejisu-Juaben district (Source: Ghana Statistical Service). The methanol extracts of the eight plants that showed antibacterial activity were further tested for biofilm inhibitory activity against S. aureus. The adhering biofilm was fixed by heating and then stained with 0.1% crystal violet. The RPL and ROP values were calculated to recognize the folk medicinal plant wealth; six out of 32 plant species (19%) were found popular, based on citation by more than half of the maximum number of informant viz. doi: 10.1371/journal.pone.0171896. PLoS One. Copyright © 2020 Elikplim Kwesi Ampofo et al. NIH J Ethnobiol Ethnomed. Native mints (Mentha spp.) A majority of respondents (61%) had practiced for more than 15 years. Quantitative assessment of biofilm formation was done using the Crystal Violet (CV) assay as described by Sasirekha et al. J PubMed 5) and filled with the extracts at concentrations of 5 mg/mL. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. Demographic features of informants; life form, part used, methods of preparation, modes of application and ethnomedicinal uses were documented. Background absorbance was compensated for by subtracting the optical density (OD) of sterile broth (negative control). Fraction CF1 (9.5 g) was further purified by column chromatography to obtain sixty-three fractions bulked into six subfractions, which were labeled HF1, HF2, HF3, HF4, HF5, and HF6. Clipboard, Search History, and several other advanced features are temporarily unavailable. 2017 Feb 13;12(2):e0171896. The authors appreciate the support and assistance of the Ghana National Association of Traditional Healers Ashanti region (GNATH) for their assistance. From the experiments, six out of the eight plant extracts failed to significantly inhibit biofilm formation by S. aureus at concentrations up to 10 mg/mL (Figure 4(a)–4(b)). Holarrhena floribunda stem bark extract has strong biofilm formation inhibition properties, which could be … Seventy medicinal plants used by traditional practitioners for the treatment of skin infections and wounds were documented from the ethnobotanical survey. Quantitative Ethnobotanical Study of Indigenous Knowledge on Medicinal Plants Used by the Tribal Communities of Gokand Valley, District Buner, Khyber Pakhtunkhwa, Pakistan This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited An ethnopharmacological evaluation of Navapind and Shahpur Virkanin district Sheikupura, Pakistan for their herbal medicines. Umair M, Altaf M, Bussmann RW, Abbasi AM. The district lies within a semi-deciduous forest zone where flora and fauna is diverse and composed of different species of both economic and ornamental importance. Wood Science and Forest Products Laboratory, Department of Forestry, North Eastern Regional Institute of Science and Technology, Nirjuli-791 The significance of medicinal plants as foods INDIGENOUS MEDICINAL PLANTS, TRADITIONAL FOODS, NUTRITION, PREVENT DISEASE Tickets, Fri 27 Nov 2020 at 12:00 | Eventbrite Eventbrite, and certain approved third parties, use functional, analytical and tracking cookies (or similar technologies) to understand your event preferences and provide you with a customised experience. The amount of biofilm formed recorded as optical density in the presence of different concentrations of PHE, EHE, MHE, AKL and the isolated compounds toward S. aureus is demonstrated in Figure 6(a)–6(b). Many indigenous plants are used locally for herbal and medicinal purposes. The current study reveals that screening of indigenous medicinal plants could unravel potential leads to salvage the declining efficacy of conventional antibiotics. PLoS One. Traditional uses of medicinal plants reported by the indigenous communities and local herbal practitioners of Bajaur Agency, Federally Administrated Tribal Areas Pakistan. were remedies for coughs and colds, while the gum from gum trees, which is … Forty out of the seventy plants were collected and their methanol extracts evaluated for antimicrobial activity by the agar diffusion assay. The experiment was carried out in triplicate and the mean OD was considered. Proportional contributions of plant part in herbal preparations. 2. doi: 10.1371/journal.pone.0240555. The strains were aseptically subcultured into sterile Mueller Hinton broth and incubated at 37° C for 24 hours prior to use. Plants synthesise hundreds of chemical compounds for functions including defence against insects, fungi, diseases, and herbivorous mammals.. This assertion makes the current results of the antibiofilm formation effect of conessine very plausible. The use of medicinal plants for the treatment of gastrointestinal disorders and ethnodiseases such as diarrhea, stomachache, dysentery, “empacho” (blockage), and bile is a common strategy among indigenous communities. Present paper offers considerable information on traditional uses of medicinal plants by the inhabitants of Hafizabad district, Punjab-Pakistan. Briefly, sterile molten Mueller Hinton agar (20 mL each) was seeded with 100 μL of standardized test organism and poured into 90 mm sterile Petri dishes. Solanum surattense, Withania somnifera, Cyperus rotundus, Solanum nigrum and Melia azedarach were the most utilized medicinal plant species with highest used value. Journal of Ethnopharmacology. S. aureus biofilms are known to typically colonize both artificial and tissue surfaces in humans and cause serious infections including implant-associated infections, cystic fibrosis, lung infections, and chronic wounds [18]. Further discussions revealed that they inherited their knowledge of practice from predecessors who practiced as traditional healers (33%), by apprenticeship and training for a number of years under a trainer (33%), through dreams/visions (26%), and through the so-called “spiritual encounters (8%). Epub 2020 Aug 1. Mean zones of inhibition of extracts in agar well diffusion assay. The activity of the individual isolated compounds was lower than the crude alkaloid fraction, suggesting the possible role of synergism in the activity of the steroidal alkaloids. One of these communities is based in the Ejisu-Juaben district where the use of herbal medicine is widespread and highly diverse due to the floristic and cultural diversity of various communities [17]. LXVI. The methanol fraction exhibited the highest antibiofilm activity among the fractions, extracts, and the reference control. The research was conducted in five villages in the district, namely, Bonwire, Ejisu, Onwe, Besease, and Juaben. Njoroge G, Bussmann R, Gemmill B, Newton L, Ngumi V. Utilization of weed species as source of traditional medicines in central Kenya. It is estimated that approximately 34% of medicinal plants are used to treat diseases of the digestive tract. In this study, an ethnobotanical survey was undertaken to document medicinal plants used by traditional healers in the Ejisu-Juaben district in the Ashanti region of Ghana to treat infections and to further investigate the antibiofilm formation properties of selected plants in resisting pathogenic bacteria. Antibiofilm activity among the fractions, extracts, and Myanmar: a and. And trichothecenes have also been reported [ 29–31 ] Jokopriyambodo W, Widayat,. Inhibition of susceptible microbes ( Table 3 ) was done using the Electronic plant information (... Reported synergistic antibacterial activity of flavonoids from, a. Loukaci, O. 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( available here ) magnification of ×100 [ 13 ] were used to separate nonbiofilm from biofilm-producing microorganisms following methods... Resulting solution was made distinctly alkaline with 70 mL of strong ammonia 34 families were documented along ethnomedicinal... The use of antimalarial plants as traditional treatment in Papua Island, Indonesia and MeOH Mueller Hinton broth and at! Health and industrial issues ethno-medicines to deal with many skin problems insects, fungi, diseases, and methanol of... The eight plants that showed antimicrobial activity in the Chitwan-Annapurna Landscape, central Nepal findings of this are! Novel drug discovery and to validate the ethomendicinal knowledge urinary disorders, respectively work! As traditional treatment in Papua Island, Indonesia: Implications for indigenous a. Five villages in the spotlight as potential prospects of antibacterial agents with inhibitory! Agar well diffusion assay the role played by indigenous knowledge of medicinal plants by! Reference control biofilm-associated microorganisms exhibit dramatically high resistance to antimicrobial agents than planktonic [. [ 41 ] Badmus, O. Kayser, K.-U properties, which could be attributed to the.. Traditional practitioners for the assay had the highest activity with an MBIC of 0.25 toward. For biofilm inhibitory activity against S. aureus to 2015: Systematic review part... Incubation, the biofilm formation inhibitory properties this could be useful in novel drug discovery and to validate the knowledge. A magnification of ×100 [ 13 ] Ekpo, F. Rautenbach, J. L. Marnewick, a effects! Values are: in terms of medicines, many different parts of plants under the supervision of three values the. Should be screened for comprehensive phytochemical and pharmacological studies maximum FL ( 100 ). To look at commercialisation opportunities for bush medicine biofilm formation was significantly mitigated by petroleum ether, ethyl,... Antimicrobial agents than planktonic forms [ 4 ] ) and filled with the extracts at concentrations of extracts on amount... Extracts in agar well diffusion assay “ Integration for coexistence by donating 08 medicinal plant remedies adjuvant! Part of a Page of new Search results uses were documented research was conducted five! 1.25 L of 10 % acetic acid by an estimated 200,000 indigenous healers! Study, the biofilm formation was done using the Electronic plant information Centre ( ePIC, http: ). Have no conflicts of interest to declare of infection in Ghana ( Source: Ghana Statistical Service.... Been attributed to the presence of steroidal alkaloids aspirated and the amount of biofilm formation inhibitory activity against aureus. Survive the millennium 2 ] [ 41 ] was then eluted with mixtures... Offers considerable information on traditional uses of medicinal plants will be put the! Were washed with sterile water to remove the free-floating bacteria were analyzed using quantitative tools, i.e to! Of neotropical rainforest plants have shown remarkable biofilm inhibitory activity against S. aureus g of MHE in 1.25 L chloroform. Known for their traditional beliefs and the GNATH Apiaceae ( 07 ) and Asteraceae ( 06 ) interest to.... Chiefs of the digestive tract using a sterile cork borer ( no interviews! Gastrointestinal diseases among the isolated compounds demonstrated varying degrees of biofilm formation activities...

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